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KMID : 0613820170270111262
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Journal of Life Science
2017 Volume.27 No. 11 p.1262 ~ p.1268
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The Hepatoprotective Effects of Hep G2 Cells and the Alcohol-metabolizing Enzyme Activities of Lemon-myrtle (Backhousia citriodora) Leaf Extracts
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Jung Kyong-Im
Kim Pan-Kil
Gal Sang-Wan
Choi Young-Ju
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Abstract
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Lemon myrtle (Backhousia citriodora), a plant in the Myrtaceae family, is native to the semitropical rainforests of Queensland and is presumably the most commercialized native spice. In Australian thousands of lemon-myrtle trees are under tillage. This study was carried out to investigate the alcohol metabolism, hepatoprotective effects and antidiabetic, tyrosinase inhibitory activity of hot-water (LMW) and 80% ethanol (LME) extracts from lemon-myrtle leaves. The alpha-glucosidase (α-glucosidase) inhibitory activities of the LMW and LME extracts were 7.66% and 40.29% at 1 mg/ml (p<0.05), respectively. The tyrosinase inhibitory activity of the LME extract was about 38.26 % at 1 mg/ml. The effects the LMW and LME extracts had on alcohol-metabolizing activities were determined by measuring the generation of reduced nicotinamide-adenine dinucleotide (NADH) by acetaldehyde dehydrogenase (ALDH) and alcohol dehydrogenase (ADH). The ADH activities of the LMW and LME extracts significantly increased in a dose-dependent manner and were about 154.40% and 192.03% at 1 mg/ml, respectively (p<0.05). The ALDH activities of the LMW and LME extracts also significantly increased in a dose-dependent manner and were about 151.14% and 192.34% at 1 mg/ml, respectively (p<0.05). At 100 μg/ml, the LMW and LME extracts showed significant protective effects against tacrine-induced cytotoxicity in Hep G2 cells. The results suggested that Backhousia citriodora leaf extracts have the potential to be significant sources for natural health products.
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KEYWORD
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Acetaldehyde dehydrogenase, alcohol dehydrogenase, α-glucosidase, hepatoprotective, lemon myrtle
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